師資
田瑞軍博士，南方科技大學(xué)化學(xué)系講席教授、國(guó)家杰出青年科學(xué)基金獲得者、化學(xué)生物學(xué)與組學(xué)分析研究中心主任、南方科技大學(xué)技術(shù)轉(zhuǎn)移中心主任、南方科技大學(xué)光明高等研究院副院長(zhǎng)、南方科技大學(xué)第一附屬醫(yī)院兼職教授；中國(guó)蛋白質(zhì)組組織CNHUPO副理事長(zhǎng)、中國(guó)化學(xué)會(huì)色譜專(zhuān)業(yè)委員會(huì)副主任委員、中國(guó)質(zhì)譜學(xué)會(huì)理事和中國(guó)分子系統(tǒng)生物學(xué)學(xué)會(huì)理事。2008年在中國(guó)科學(xué)院大連化學(xué)物理研究所獲得分析化學(xué)博士學(xué)位。先后在加拿大渥太華大學(xué)Ottawa Institute of Systems Biology和多倫多大學(xué)/西奈山醫(yī)院Lunenfeld-Tanenbaum Research Institute完成博士后研究。2014年起受聘南方科技大學(xué)化學(xué)系，致力于蛋白質(zhì)組學(xué)的方法學(xué)和技術(shù)研究，并強(qiáng)調(diào)其在細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)和胰腺癌腫瘤微環(huán)境等生物醫(yī)學(xué)研究方向的應(yīng)用。已在國(guó)內(nèi)外主流學(xué)術(shù)期刊Nature、Nat. Chem. Biol.、Cell Chem. Biol.、PNAS、Nat. Commun.、Mol. Cell. Proteomics、Anal. Chem.等上發(fā)表論文百余篇。曾榮獲由國(guó)際蛋白質(zhì)結(jié)構(gòu)分析和蛋白質(zhì)組學(xué)協(xié)會(huì)（IAPSAP）頒發(fā)的2012 Young Investigator Award、廣東省杰出青年基金和深圳市鵬城學(xué)者特聘教授等。主持國(guó)家科技部重點(diǎn)研發(fā)計(jì)劃、國(guó)家自然科學(xué)基金委重大研究計(jì)劃集成項(xiàng)目、深圳市功能蛋白質(zhì)組學(xué)重點(diǎn)實(shí)驗(yàn)室等項(xiàng)目。曾擔(dān)任中國(guó)化學(xué)會(huì)第23屆全國(guó)色譜學(xué)術(shù)報(bào)告會(huì)共同執(zhí)行主席、第5屆中加系統(tǒng)生物學(xué)研討會(huì)和第2屆中國(guó)蛋白質(zhì)組組織（CNHUPO）青年學(xué)者研討會(huì)共同主席等。擔(dān)任分析化學(xué)、色譜雜志編委和Curr. Opin. Chem. Biol. Guest Editor。
代表性承擔(dān)項(xiàng)目：
1. 國(guó)家科技部-國(guó)家重點(diǎn)研發(fā)計(jì)劃，組織微環(huán)境蛋白質(zhì)組可視化的前沿技術(shù)，2024.12-2029.11，項(xiàng)目負(fù)責(zé)人
2. 國(guó)家自然科學(xué)基金-重大研究計(jì)劃，腫瘤微環(huán)境中關(guān)鍵蛋白質(zhì)復(fù)合物動(dòng)態(tài)修飾的多維度發(fā)現(xiàn)與表征，2023.01-2025.12，項(xiàng)目負(fù)責(zé)人
3. 國(guó)家自然科學(xué)基金-杰出青年科學(xué)基金項(xiàng)目，22125403，復(fù)雜生物樣品分離與質(zhì)譜分析，2022.01-2026.12，項(xiàng)目負(fù)責(zé)人
4. 深圳市功能蛋白質(zhì)組學(xué)重點(diǎn)實(shí)驗(yàn)室，2024.02-2026.02，項(xiàng)目負(fù)責(zé)人
6. 深圳市科創(chuàng)局-重點(diǎn)項(xiàng)目，基于多組學(xué)的腫瘤耐藥新靶點(diǎn)篩選及分子機(jī)制研究，2025.03-2028.03，項(xiàng)目負(fù)責(zé)人
代表性論文：
Invited reviews:
1. P. W. Huang,? W. N. Gao,? C. Y. Fu,? R. J. Tian,* Functional and clinical proteomic exploration of pancreatic cancer, Mol. Cell. Proteomics, 2023, 22, 100575. (Invited Reviews)
2. Z. Y. Liu,? X. Chen,? S. R. Yang, R. J. Tian,* F. J. Wang,* Integrated mass spectrometry strategy for functional protein complex discovery and structural characterization, Curr. Opin. Chem. Biol., 2023, 74, 102305. (Invited Reviews)
3. Y. H. Mao, X. Wang, P. W. Huang, R. J. Tian,* Spatial proteomics for understanding the tissue microenvironment, Analyst, 2021, 146, 3777-3798. (Invited Reviews)
4. J. N. Zheng,? X. Chen,? Y. Yang, C. S. H. Tan, R. J. Tian,* Mass spectrometry-based protein complex profiling in time and space, Anal. Chem., 2021, 93, 598–619. (Invited Reviews)
5. X. T. Ye,? J. Tang,? Y. H. Mao,? X. Lu, Y. Yang, W. D. Chen, X. Y. Zhang, R. L. Xu, R. J. Tian,* Integrated proteomics sample preparation and fractionation: method development and applications, TrAC Trends Anal. Chem., 2019, 120, 115667. (Highlighted as Front Cover)
6. R. J. Tian,* Exploring intercellular signaling by proteomic approaches, Proteomics, 2014, 14, 498-512. (Invited Reviews)
Research articles:
1. Y. H. Mao,? Y. Li,? Z. D. Zheng,? Y. F. Xu,? M. Ke, A. He, F. C. Liang, K. R. Zhang, X. Wang, W. N. Gao, R. J. Tian,* All-at-once spatial proteome profiling of complex tissue context with single-cell-type resolution by proximity proteomics, Cell Systems, 2025 May 5:101291.
2. P. W. Huang,? W. N. Gao,? C. Y. Fu,? M. Wang,? Y. G. Li,? B. Z. Chu, A. He, Y. Li, X. M. Deng, Y. H. Zhang, Q. Kong, J. X. Yuan, H. B. Wang, Y. Shi,* D. Gao,* R. Y. Qin,* T. Hunter, R. J. Tian,* Clinical functional proteomics of intercellular signaling in pancreatic cancer, Nature, 2025, 637, 726–735.
3.Y. F. Xu,? X. Wang,? Y. Li,? Y. H. Mao,? Y. R. Su, Y. Z. Mao, Y. Yang, W. N. Gao, C. Y. Fu, W. D. Chen, X. T. Ye, F. C. Liang, P. Z. Bai, Y. Sun, S. P. Li, R. L. Xu, R. J. Tian,* Multimodal single cell-resolved spatial proteomics reveal pancreatic tumor heterogeneity, Nat. Commun., 2024, 15, 10100.
4. Y. H. Mao,? Y. Chen,? Y. Li,? L. D. Ma,? X. Wang, Q. Wang, A. He, X. Liu, T. Y. Dong, W. N. Gao, Y. F. Xu, L. Liu, L. Ren, Q. Liu, P. Zhou, B. Hu, Y. W. Zhou,* R. J. Tian,* Z. L. Shi,* Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury, Cell Reports, 2024, 43, 2,113689.
5. Q. Wu,? J. N. Zheng,? X. T. Sui,? C. Y. Fu,? X. Z. Cui, B. Liao, H. C. Ji, Y. Luo, A. He, X. Lu, C. S. H. Tan,* R. J. Tian,* High-throughput drug target discovery by fully automated proteomics sample preparation platform, Chemical Science, 2024, 15, 2833-2847.
6.Y. C. Weng,? W. D. Chen,? Q. Kong,? R. X. Wang, R. X. Zeng, A. He, Y. J. Liu, Y. H. Mao, Y. Q. Qin, W. S. C. Ngai, H. Zhang, M. Ke, J. Wnag, R. J. Tian,* P. R. Chen,* DeKinomics pulse-chases kinase functions in living cells, Nat. Chem. Biol., 2024, 20, 615-623.
7. J. N. Zheng,?* Z. D. Zheng,? C. Y. Fu,? Y. C. Weng, A. He, X. T. Ye, W. N. Gao, R. J. Tian,* Deciphering intercellular signaling complexes by interaction-guided chemical proteomics, Nat. Commun., 2023, 14, 4138.
8. X. Chen,? S. P. Ji,? Z. Y. Liu, X. Yuan, C. S. Xu, R. X. Qi, A. He, H. Zhao, H. P. Song, C. L. Xiao, W. N. Gao, P. Chen, R. Luo, P. F. Li,* F. J. Wang,* X. M. Yang, R. J. Tian,* Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics, Cell Chem. Biol., 2022, 29, 1024-1036.
9. M. Ke,? X. Yuan,? A. He, P.Y. Yu, W.D. Chen, Y. Shi, T. Hunter, P. Zou, R. J. Tian,* Spatiotemporal Profiling of Cytosolic Signaling Complexes in Living Cells by Selective Proximity Proteomics, Nat. Commun., 2021, 12, 71.
10. Y. Shi,* W. N. Gao, N. K. Lytle, P. W. Huang, X. Yuan, A. M. Dann, M. Ridinger, K. E. DelGiorno, G. Erikson, H. Y. Sun, J. Meisenhelder, E. Terenziani, P. Santisakultarm, U. Manor, R. L. Xu, M. Leblanc, S. E. Umetsu, E. A. Collisson, A. M. Lowy, T. Reya, T. R. Donahue, M. Downes, G. M. Wahl, R. M. Evans, T. Pawson, R. J. Tian,* T. Hunter,* Targeting LIF-mediated paracrine interaction for pancreatic cancer therapy and monitoring, Nature, 2019, 569, 131-135.
11. B. Z. Chu,? A. He,? Y. T. Tian, W. He, P. Z. Chen, J. T. Hu, R. L. Xu, W. B. Zhou, M. J. Zhang, P. Y. Yang, S. S. C. Li, Y. Sun,* P. F. Li,* T. Hunter, R. J. Tian,* Photoaffinity-engineered protein scaffold for systematically exploring Native phosphotyrosine signaling complexes in tumor samples. Proc. Natl. Acad. Sci. U. S. A., 2018, 115, E8863-E8872.
12. R. J. Tian,*? H. P. Wang,? G. D. Gish, E. Petsalaki, A. Pasculescu, Y. Shi, M. Mollenauer, R. D. Bagshaw, N. Yosef, T. Hunter, A. Gingras, A. Weiss,* T. Pawson, A combinatorial proteomic analysis of intercellular signaling applied to the CD28 T cell co-stimulatory receptor, Proc. Natl. Acad. Sci. U. S. A. , 2015, 112, E1594–E1603.
13. Q. Kong,? M. Ke,? Y. C. Weng, Y. Q. Qin, A. He, P. F. Li,* Z. W. Cai,* R. J. Tian,* Dynamic phosphotyrosine-dependent signaling profiling in living cells by two-dimensional proximity proteomics, J. Proteome Res., 2022, 21, 2727–2735.
14. Q. Kong,? Y. C. Weng,? Z. D. Zheng, W. D. Chen, P. F. Li,* Z. W. Cai,* R. J. Tian,* Integrated and high-throughput approach for sensitive analysis of tyrosine phosphoproteome, Anal. Chem., 2022, 94, 40, 13728–13736.
15. Y. H. Mao,? P. Z. Chen,? M. Ke, X. Chen, S. P. Ji, W. D. Chen, R. J. Tian,* Fully integrated and multiplexed sample preparation technology for sensitive interactome profiling, Anal. Chem., 2021, 93, 5, 3026–3034.
16. P. W. Huang, Q. Kong, W. N. Gao, B. Z. Chu, H. Li, Y. H. Mao, Z. W. Cai, R. L. Xu, R. J. Tian,* Spatial proteome profiling by immunohistochemistry-based laser capture microdissection and data-independent acquisition proteomics, Anal. Chim. Acta, 2020, 1127, 140-148.
17. Q. Kong,? P. W. Huang,? B. Z. Chu, M. Ke, W. D. Chen, Z. D. Zheng, S. P. Ji, Z. W. Cai, P. F. Li, and R. J. Tian,* High-throughput and integrated chemical proteomic approach for profiling phosphotyrosine signaling complexes, Anal. Chem., 2020, 92, 8933-8942.
18. X. Lu,? Z. K. Wang,? Y. Gao, W. D. Chen, L. J. Wang, P. W. Huang, W. N. Gao, M. Ke, A. He, and R. J. Tian,* Autoproteome chip system for fully automated and integrated proteomics sample preparation and peptide fractionation, Anal. Chem., 2020, 92, 8893-8900.
19. P. W. Huang, H. J. Li, W. N. Gao, Z. W. Cai,* R. J. Tian,* A fully integrated spintip-based approach for sensitive and quantitative profiling of region-resolved in vivo brain glycoproteome, Anal. Chem., 2019, 91, 9181-9189.
20. R. L. Xu,? J. Tang,? Q. T. Deng, W. He, X. J. Sun, L. Xia, Z. Q. Cheng, L. S. He, S. Y. You, J. T. Hu, Y. X. Fu, J. Zhu, Y. X. Chen, W. N. Gao, A. He, Z. Y. Guo, L. Lin, H. Li, C. F. Hu, R. J. Tian,* Spatial-resolution cell type proteome profiling of cancer tissue by fully integrated proteomics technology, Anal. Chem., 2018, 90, 5879?5886.
21. W. D. Chen, S. Wang, S. Adhikari, Z. H. Deng, L. J. Wang, L. Chen, M. Ke, P. Y. Yang, R. J. Tian,* Simple and integrated spintip-based technology applied for deep proteome profiling, Anal. Chem., 2016, 88, 4864-4871.